FLUORESCENCE 2D AND 3D SPECTRA ANALYSIS OF TRYPTOPHAN, TYROSINE AND PHENYLALANINE

Authors

  • K.R. Grigoryan Chair of Physical and Colloids Chemistry, YSU, Armenia
  • H.A. Shilajyan Chair of Physical and Colloids Chemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2017.51.1.003

Keywords:

tryptophan, tyrosine, phenylalanine, fluorescence, 2D, 3D spectra

Abstract

The analysis of fluorescence 2D and 3D spectra of tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe) measured in physiological solution at 293 K are presented. The spectral characteristics of 2D fluorescence spectra of amino acids (AAc) are recorded in the following conditions: for Trp λex/λem=280/357, for Tyr 275/303 and for Phe 257/283 nm, and the 3D spectra of AAc recorded in λex=200–450 and λem=200–500 nm ranges are given. It has been revealed that 3D spectra of AAc are characterized by two strong excitation/emission peaks located at λex/λem=230/350 and 280/350 nm. The high energy emission (absorption) at 230 nm is due to n→π* or π→π* transitions of the carboxylic or amide group and low energy emission (absorption) at 280 nm is due to π→π* transition in the indole ring. In 3D spectra Rayleigh scattering peak and second-order scattering peak are identified as well.

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Published

2017-03-30

How to Cite

Grigoryan, K.R., and H.A. Shilajyan. 2017. “FLUORESCENCE 2D AND 3D SPECTRA ANALYSIS OF TRYPTOPHAN, TYROSINE AND PHENYLALANINE”. Proceedings of the YSU B: Chemical and Biological Sciences 51 (1 (242):3-7. https://doi.org/10.46991/PYSU:B/2017.51.1.003.

Issue

Vol. 51 No. 1 (242) (2017)

Section

Chemistry

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Copyright (c) 2017 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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