STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.A. Parsadanyan Chair of Biophysics, YSU, Armenia
  • V.G. Sahakyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2017.51.2.100

Keywords:

DNA, ethidium bromide, methylene blue, acridine orange, differential spectra, isosbestic point, pseudoisosbestic point

Abstract

Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and AO by intercalation and electrostatic modes. It was shown that the simultaneous performance of intercalation and semiintercalation modes condition the formation of a real isosbestic point (IP) in the spectra of EtBr–DNA complexes. In the absence of intercalation at MB interaction with DNA a pseudo-IP in differential absorption spectra of those complexes is formed. In the spectra of AO–DNA complexes both IP and pseudo-IP are not formed, despite the fact that this ligand binds to DNA by intercalation mode.

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Published

2017-06-13

How to Cite

Antonyan, A.P., M.A. Parsadanyan, and V.G. Sahakyan. 2017. “STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA”. Proceedings of the YSU B: Chemical and Biological Sciences 51 (2 (243):100-105. https://doi.org/10.46991/PYSU:B/2017.51.2.100.

Issue

Section

Biology