STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA
DOI:
https://doi.org/10.46991/PYSU:B/2017.51.2.100Keywords:
DNA, ethidium bromide, methylene blue, acridine orange, differential spectra, isosbestic point, pseudoisosbestic pointAbstract
Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and AO by intercalation and electrostatic modes. It was shown that the simultaneous performance of intercalation and semiintercalation modes condition the formation of a real isosbestic point (IP) in the spectra of EtBr–DNA complexes. In the absence of intercalation at MB interaction with DNA a pseudo-IP in differential absorption spectra of those complexes is formed. In the spectra of AO–DNA complexes both IP and pseudo-IP are not formed, despite the fact that this ligand binds to DNA by intercalation mode.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Proceedings of the YSU
![Creative Commons License](http://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.