STUDY OF COMPARATIVE INTERACTION OF ETHIDIUM BROMIDE AND MITOXANTRONE WITH NUCLEIC ACIDS

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.A. Torosyan Chair of Biophysics, YSU, Armenia
  • M.A. Parsadanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2018.52.1.052

Keywords:

DNA, ethidium bromide, mitoxantrone, poly(rA)-poly(rU), melting parameters

Abstract

The study of joint binding of intercalating compounds – ethidium bromide (EtBr) and mitoxantrone (MTX) with double-stranded DNA (ds-DNA) and poly(rA)-poly(rU) (ds-RNA) has been carried out by UV-melting method. The melting parameters of the complexes of both EtBr–ds-DNA–MTX and EtBr–ds-RNA–MTX triple systems were obtained. Changes of the melting parameters of these complexes revealed that EtBr binds to both ds-DNA and ds-RNA by several modes, though these binding modes of EtBr with nucleic acids (NA) were performed in the presence of MTX as well. MTX binds to ds-DNA and ds-RNA by semi-intercalation mode although this ligand shows more pronounced affinity to DNA than to RNA. It was found that at the joint binding of both ligands to NA a competition emerges between them and the effect of EtBr on the melting parameters of NA prevails.

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Published

2018-03-15

How to Cite

Antonyan, A.P., M.A. Torosyan, and M.A. Parsadanyan. 2018. “STUDY OF COMPARATIVE INTERACTION OF ETHIDIUM BROMIDE AND MITOXANTRONE WITH NUCLEIC ACIDS”. Proceedings of the YSU B: Chemical and Biological Sciences 52 (1 (245):52-57. https://doi.org/10.46991/PYSU:B/2018.52.1.052.

Issue

Section

Biology