BOVINE SERUM ALBUMIN FLUORESCENCE QUENCHING BY TANNIC ACID IN DIMETHYLSULFOXIDE CONTAINING SOLUTIONS

Authors

  • K.R. Grigoryan Chair of Physical and Colloids Chemistry, YSU, Armenia
  • H.A. Shilajyan Chair of Physical and Colloids Chemistry, YSU, Armenia
  • V.A. Hovhannisyan Chair of Physical and Colloids Chemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2020.54.2.099

Keywords:

bovine serum albumin, tannic acid, modified Stern-Volmer equation, dimethylsulfoxide, fluorescence, sphere of action

Abstract

Bovine serum albumin (BSA) interaction with tannic acid (TA) has been studied in dimethylsulfoxide (DMSO) aqueous solutions at different temperatures (293 and 303 K). To find out the fluorescence quenching mechanism of BSA in the presence of TA, the fluorescence data were analyzed according to the modified Stern-Volmer equation based on the approach of the existence of a “sphere of action” (a type of apparent static quenching). The values of apparent static and bimolecular quenching constants were calculated. The effect of DMSO and temperature on BSA–TA interactions is explained on the basis of structural changes in the “sphere of action” of the fluorophore due to the possible inclusion of DMSO molecules in this sphere.

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Published

2020-08-17

How to Cite

Grigoryan, K.R., H.A. Shilajyan, and V.A. Hovhannisyan. 2020. “BOVINE SERUM ALBUMIN FLUORESCENCE QUENCHING BY TANNIC ACID IN DIMETHYLSULFOXIDE CONTAINING SOLUTIONS”. Proceedings of the YSU B: Chemical and Biological Sciences 54 (2 (252):99-104. https://doi.org/10.46991/PYSU:B/2020.54.2.099.

Issue

Vol. 54 No. 2 (252) (2020)

Section

Chemistry

License

Copyright (c) 2020 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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