ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)

Authors

  • Mariam A. Shahinyan Chair of Biophysics, YSU, Armenia
  • Zvart H. Movsisyan Chair of Biophysics, YSU, Armenia
  • Gohar G. Hovhannisyan Chair of Biophysics, YSU, Armenia
  • Poghos H. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2021.55.2.151

Keywords:

synthetic polynucleotide, methylene blue, acridine orange, absorption spectrum, fluorescence spectrum

Abstract

The interaction of methylene blue (MB) and acridine orange (AO) with poly(rA)-poly(rU) was studied using absorption and fluorescence spectroscopy methods. The absorption and fluorescence spectra of complexes of these ligands with a polynucleotide were obtained, similar to those of complexes of these ligands with double-stranded (ds-) DNA. It was revealed that the isosbestic point in the spectra of the AO-poly(rA)-poly(rU) and MB-poly(rA)-poly(rU) complexes is not formed, although the binding of AO and MB with ds-RNA by intercalation mode is not excluded.

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Published

2021-08-30

Issue

Vol. 55 No. 2 (255) (2021)

Section

Biology

License

Copyright (c) 2021 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)”. 2021. Proceedings of the YSU B: Chemical and Biological Sciences 55 (2 (255): 151-57. https://doi.org/10.46991/PYSU:B/2021.55.2.151.

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