ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)

Authors

  • Mariam A. Shahinyan Chair of Biophysics, YSU, Armenia
  • Zvart H. Movsisyan Chair of Biophysics, YSU, Armenia
  • Gohar G. Hovhannisyan Chair of Biophysics, YSU, Armenia
  • Poghos H. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2021.55.2.151

Keywords:

synthetic polynucleotide, methylene blue, acridine orange, absorption spectrum, fluorescence spectrum

Abstract

The interaction of methylene blue (MB) and acridine orange (AO) with poly(rA)-poly(rU) was studied using absorption and fluorescence spectroscopy methods. The absorption and fluorescence spectra of complexes of these ligands with a polynucleotide were obtained, similar to those of complexes of these ligands with double-stranded (ds-) DNA. It was revealed that the isosbestic point in the spectra of the AO-poly(rA)-poly(rU) and MB-poly(rA)-poly(rU) complexes is not formed, although the binding of AO and MB with ds-RNA by intercalation mode is not excluded.

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Published

2021-08-30

How to Cite

Shahinyan, Mariam A., Zvart H. Movsisyan, Gohar G. Hovhannisyan, and Poghos H. Vardevanyan. 2021. “ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)”. Proceedings of the YSU B: Chemical and Biological Sciences 55 (2 (255):151-57. https://doi.org/10.46991/PYSU:B/2021.55.2.151.

Issue

Vol. 55 No. 2 (255) (2021)

Section

Biology

License

Copyright (c) 2021 Proceedings of the YSU

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