ROLE OF GLYCINE-BETAINE IN THE GROWTH AND HYDROGENASES ACTIVITY OF $RALSTONIA~EUTROPHA$ H16

Authors

  • Meri K. Iskandaryan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2023.57.2.154

Keywords:

glycine-betaine, Ralstonia eutropha H16, [NiFe]-hydrogenases

Abstract

Glycine-betaine (GB) is an abundant organic material in soil, and the chemo­litho­autotrophic soil bacterium R. eutropha H16, one of the best bio-technological research models, may involve it in metabolism. The effect of different concentrations of GB (7–300 µmol/mL) on bacterial growth parameters and H2-oxidizing activity of hydrogenases (Hyd) was studied. Stimulation of bacterial growth was registered at GB low concentrations, however, high concentrations have a partial inhibitory effect, in contrast to control (Fructose-Nitrogen medium). Upon GB supplemen­tation, the min and max H2-oxidizing Hyd activity of R. eutropha whole cells were 3.4±0.01 U (g CDW)−1 and 16.4±0.01 U (g CDW)−1, respectively at concentrations of 7 µmol/mL and 300 µmol/mL, while it is absent in the control. These results might be used to develop new approaches to produce oxygen-tolerant Hyd.

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Published

2023-07-07

How to Cite

Iskandaryan, Meri K. 2023. “ROLE OF GLYCINE-BETAINE IN THE GROWTH AND HYDROGENASES ACTIVITY OF $RALSTONIA~EUTROPHA$ H16”. Proceedings of the YSU B: Chemical and Biological Sciences 57 (2 (261):154-63. https://doi.org/10.46991/PYSU:B/2023.57.2.154.

Issue

Vol. 57 No. 2 (261) (2023)

Section

Biology

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Copyright (c) 2023 Proceedings of the YSU

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