COMPARATIVE ANALYSIS OF THE EFFECTS OF PLATINUM COMPOUNDS ON THERMOSTABILITY OF DNA

I.E. Grigoryan; Ye.B. Dalyan; S.G. Haroutiunian

doi:10.46991/PYSU:A/2011.45.3.052

COMPARATIVE ANALYSIS OF THE EFFECTS OF PLATINUM COMPOUNDS ON THERMOSTABILITY OF DNA

Authors

I.E. Grigoryan Chair of Molecular Physics, YSU, Armenia
Ye.B. Dalyan Chair of Molecular Physics, YSU, Armenia
S.G. Haroutiunian Chair of Molecular Physics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:A/2011.45.3.052

Keywords:

DNA, binuclear compound of bivalent platinum, cis-DDP, renaturation, DNA melting, interstrand cross-link

Abstract

The thermal denaturation profiles of Calf Thymus DNA in the presence of binuclear compounds of bivalent platinum (cis-[{Pt(NH₃)₂Cl}₂Pyr]Cl₂) were studied by absorption spectrophotometry and differential adiabatic scanning microcalorimetry. A comparison with an active antitumor drug cis-diamminedichloroplatinum (cis-DDP) was made. Experimental data witness that small concentrations (r<0.3) of binuclear platinum compound stabilized the structure of Calf Thymus DNA stronger than cis-DDP. Both the complexes tend to refold the denaturated DNA. It is rather interesting that in case of 10^-6 M concentration of binuclear platinum compound (r=0.03) the denaturated DNA is refolded by 77.62%, whereas under the same condition cis-DDP leads to 49.01% refolding of denaturated DNA. Such a high percent of renaturation was probably the result of interstrand cross-links formation.

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Published

2011-10-17

How to Cite

Grigoryan, I., Dalyan, Y., & Haroutiunian, S. (2011). COMPARATIVE ANALYSIS OF THE EFFECTS OF PLATINUM COMPOUNDS ON THERMOSTABILITY OF DNA. Proceedings of the YSU A: Physical and Mathematical Sciences, 45(3 (226), 52–57. https://doi.org/10.46991/PYSU:A/2011.45.3.052