Vol. 36 No. 1 (197) (2002)

The kinetics of the accumulation of a coloured product, as well as the influence of base and acid additions on the phenyldiethanolamine-potassium persulfate reaction rate in water at 298 K have been studied. It has been established that the addition of H₂SO₄ slows down the reaction, which is result of amine's molecule binding to the corresponding salt. The base's action nature on the rate of the studied reaction differs from cases described in literature concerning amine-peroxide reactions. The obtained data are explained assuming that in presence of NaOH the nature of formed aminyl radicals is changed. It means that NaOH changes the mechanism of phenyldiethanolamine's oxidation by persulfate.

The process of separation of biomass from L-proline cultural liquid has been investigated and the optimal technological parameters of the process has been defined. Proline sorption curves at various pH values of supernatant have been obtained and pH value, at which the maximum sorption of proline takes place on cationite as defined. The proline sorption isotherm from synthetic solution and supernatant of cultural liquid on the KU-$2\times 8$ cation-exchange resin has been obtained and the coefficients of proline and accompanying amino-acids distribution has been calculated. The stationary front width of proline-ion sorption in dependence of supernatant flow rate has been studied and the optimal parameter of the process has been defined. On the basis of the data obtained, the proline sorption from cultural liquid supernatant in the industrial conditions on 4 m³ column has been carried out and proline sorption and accompanying admixture curves on KU-$2\times 8$  cation-exchange resin have been obtained. The data obtained have been used as basis technology for working out of the amino acid mixtures from the cultural liquid of proline.

An interaction of chloride anionic complex of osmium (IV) with thiazine’s row organic basic dye dimethylthionine (Azure I) has been investigated. Forming ionic associate could be extracted by dichloroethan–tetrachlorocarbon (9 : 1) binary mixture. Maximal light absorption observed at 650 nm wavelengths. The optimal acidity of aqueous phase is pH 3.0 by hydrochloric acid. The optimal concentration of basic dye could be secured by the addition of 1.2–1.8 mL of 0.05% solution of dimethylthionine. The range of determined concentration is 2.5–56.6 mcg of Os (IV) in 10 mL of aqueous phase. The value of seeming coefficient of dimethylthionine hexachloroosmiate (IV) ionic associates organic extracts molar extinction ε(650) = 1.98 10⁴ L· mol^–1·cm^–1. The molar ratio between dimethylthionine cation and hexachloroosmiate (IV) is 2 : 1.

Realized the synthesis of 1-ethoxycarbonylcyclopentanone-2 and 1-ethoxycarbonylcycloheptanone-2 through Dickmans condensation. During this reaction for diethylesthers of sebacin acid in the presence of DMFA have been received monodimethylamid of sebacin acid. In a process of interaction received ketoesthers with binucleofiles have been synthesized corresponding derivatives of pyrimidine and pyrazolone.

A convenient method for obtaining of 2,4-disubstituted-2-ethoxycarbonyl-4- pentanolids by means of cyclization using saturated sulfuric acid has been suggested. It has been shown that 3,8-dimethyl-3,8-dialkil-2,7-dioxaspiro[4,4] nonan-l,6-diones are formed as a result of cyclization of dialkenilmalonig esters. Optimal conditions of cyclization have been worked out which provide high yields of goaled products.

The amino acid composition of pig’s blood plasma proteins was investigated for the purpose of application in the parenteral nutrition. The greatest output of amino acids is observed in the case of 32 hours’ acid hydrolysis, which constitutes 6.572 g per 100 mL of blood plasma.

The influence of different components on proline oxidaze (PO) and piroline-5-carboxilate dehydrogenaze (P5KD) has been studied. In all stages of metamorphoses of the haricot beetlefly Acantoscelides obtectus Say. NaF considerably depresses PO and P5KD activity. The influence of different concentrations of citrate and adenylic nucleotides on enzymes activity of proline degradation of beetles has been investigated. The high concentrations of the stated compounds depress the activity of enzymes, which are depressed by ATF more than 87% under 10^–5M.

The activity of systems of several enzymes biosynthesis of membrane glycerolipids in lung tissues at ionizing irradiation is studied. It is revealed that irradiation brings a sharp decrease of glycerokinase and cytoplasmatic and mytochondrial glycerophosphat dehydrogenase in lungs. In those conditions, the activity of phospholipase A₂ is sharply increased in the mentioned tissue, which gives evidence about the speed of membrane phosphatides-glycerides degradation. The application of 4-butanolides is accompanied with processes of glicerokinases and glicolitics ways of phosphatidogeneses in the mentioned tissue during ionizing irradiation.

The interplay of alleles of genes of different types of lethality results in disturbance of genesis development of hybrid wheat plants, the fecundity of blossom dust and reduced productivity of wheat. It is marked depression of development of hybrids pistil. The complementation of gentle alleles of lethal genes results in minor decrease of fecundity of blossom dust and productivity of the wheat. The observed gentle depression of the studied tags can be the outcome of general delicacy of a hybrid organism.

Changes of relative volume of parenchyma and stroma as well as the processes of morphological reconstructions of reticular fibers in regenerating liver of rats and domestic hens after partial hepatectomy have been studied. It was shown that dynamics and character of changes of these parameters in hens and rats submit to the general regularities.

The advantages of the proposed colorimetric method is demonstrated in the experiments for determination of the activity of leucine-aminotransferase in pancreatic and liver tissues.

The question about protein biosynthesis in yeast cells under conditions of nitrogen starvation was investigated after reconditioning of normal nutrition. Valin-N¹⁵ exchange and engagement of N¹⁵ in summary protein and in several amino acids of yeasts Candida guilliermondii WKM U-42 has shown the unequal protein anabolism during the recondition of nitrogen nutrition of starved yeasts.

Preoperational stress resulted to significant changes of cardio dynamic parameters from a type of regulation of a cordial rhythm on a degree of expression of vegetative reactions is revealed. On the day of operation the increase of frequency of cordial contractions and hypertonical type of circulation of the blood described by authentic increase of parameters of arterial pressure and minute volume of blood was registered at 62.5% norm tonics. Hypo kinetic of hemodynamics was registered at 37.5% patients-norm tonics. Preoperational psycho emotional stress was more reflected on a cardio dynamic sympaticotonics with regulation of a cordial rhythm in a zone of a stress.

The investigation of floristic composition and vegetation of Astvatsatsin mountain revealed 250 species from 117 genera and 37 families. Identification of flora showed that the most common species belong to plant from the following families: Asteraceae, Poaceae, Fabaceae, Caryophyllaceae and Brassicacea. It has been established that the investigated area is represented by frigana, steppe, meadow and petrofits.

It is established the perspective of wide application T. viride for protection of plants from root inhabitant patogenes and utilization accumulating in soil foreign polymeric compound, used for protection against the pests of agricultural cultures.

To the previously known 16 species of Chiroptera (belonging to two families – Rhinolophidae, Vespertilionidae)  it is proposed to add 11 more new species (belonging to three families – Rhinolophidae, Vespertilionidae,  and Mollosidae). Three of these species are discovered recently.