Vol. 38 No. 2 (204) (2004)

The intermolecular interactions and solvation processes in solutions of acrylonitrile (AN) with dymetylsulfoxide (DMSO) using spectral (IR, UV, DRS) and physicochemical (viscometry, electrocunductimetry) methods have been investigated. It was shown that under the action of AN occurs destruction of assosiations of molecules DMSO, which is accompanied by partial ionizing the molecules AN and formation of new assosiations between molecules DMSO and AN. There is installed that in dymetylsulfoxide solutions AN up to 0.6 mole fraction of DMSO dominate assosiations AN. With the increase of concentrations of sulfoxide (from 0.6 up to 0.8 mole fraction) new assosiations between DMSO and AN with composition 2DMSO : AN are formed. In more concentrating sulfoxide solutions (X_DMSO>0.8) dominate round-robin dimers of DMSO. Intermolecular interactions between AN–AN, AN–DMSO and DMSO–DMSO, mainly, have dipole-dipolar nature.

IR-spectra of acrylamide are investigated at the presence of lithium and copper (Cu²⁺) chlorides, and also lithium perchlorate and nitrate in acetonitrile. It has been shown, that solvation of cations occurs due to their interaction both with oxygen of a carbonyl group, and with nitrogen of amide group. And this interaction substantially has donor-acceptor character.

Possibility of using of inorganic sorbent ion-exchanger argyllizite for thin-layer chromatographic separation of rare elements’ ions (rhenium, molybdenum, vanadium, tungsten) using mixtures of ethanol with different acid and bases solutions as mobile phase has been studied. It has been estimated that mobile phase content and concentration of its components influence considerably on migration properties of separated ions. The presence of ethanol in the mobile phase creates possibility for regulation of ions mobility and secures compactness of ions’ pots on thin layers. It has been shown that argyllizite fixed thin layers can be used successfully for separation of micro-amounts of ions of rare elements. New versions for separation of multi-component mixtures of above-mentioned ele-ments have been elaborated.

By interaction of 2-oxo-3-aIkhyl-6-substituted-l,4-dioxans with 2-oxo-3-alkhyl-6-methyl-l,4-hexacyclo-5-ens in the presence of catalytic quantities of zyncum chloride by laser radiation and room temperature dioxanonildioxanons are obtained with high yields. It has been studied the influence of c on the reaction yields.

It has been shown that under influence of antitumor antibiotic doxorubicine in combination with antistressful preparations (Diazepam, preparation-346) some characteristics of tumor DNA (5-methylcytozine, temperature and interval of melting) approximate to appropriate characteristics of DNA of control rats more closely than only under doxorubicine influence.

   The activity of enzyme glutaminaze has been investigated in the mithocondreal fraction of yeasts Candida guillermondii and parameciums Paramecium multimicronucleatum. The investigations show that phospate, bicarbonate, ATP and GTP positively can increase the activity of mithochondrial enzimes of both objects. Activating effect have some aminoacids as glicine, methionine, histidine and N-acetil triptofhane. The glutamic and aspargic acids decrease the glutaminase activity of both objects. It is also shown the modulating effect of different hormones on mithochondrial glutaminase of yeasts and parameciums as tiroxine, adrenaline, hidrocortizone. Serotonin and dexametazone show different effects on yeasts and parameciums. Both of them positively regulate the parameciums mithochondrial glutaminase, while inhibiting the yeasts glutaminase.

The aim of the present work was the study of functional condition of adolescents of gymnasium «Nersisyan» during the examionation period. Mathematical analysis of heart functional factors and hemodinamic exponents changes of pupils showed that the examination stress brings to the tension of Central Nervous system. Changes in the integral factors state the prefominace of the sympathetic part of Nervous system. The results reflect the most strained function of adolesents’ circulatory system during the examination period.

For the first time the molecular-cytogenetic method of fluorescence in situ hybridization (FISH) was widely used in Armenia in prenatal and postnatal diagnostics of 30 patients with different forms of chromosomal pathology. Application of FISH method in cytogenetic practice of Armenia increased the efficiency of diagnostics of chromosomal anomalies.

In the present time solution of actual medical problems needs a molecular biological basis. Biomedicine a new branch on the junction of such sciences as medicine and biology – is coming up into existence. Realization of «Human Genome Project» is a brilliant example of collaboration between these two big fields of science. It is possible to apply immunogenetic data in biomedicine and particularly in transplantology. Without the knowledge of histocompatibility antigens it is impossible to characterize the donor and recipient on the one hand and on the other hand databank obtained during the DNA typing gives great opportunities and invaluable information in the study of population structure.

Possibility of using thin layers of natural sorbent ion-exchanger argyllizite for quantitative determination of vanadium in solutions has been studied. When using hydrochloric acid (0.l mol /l)–ethanol 9:1 by volume mixtures as mobile phase the spots of vanadate-ions on the thin layer of the sorbent have compact strictly oval shape. This allows to suggest a new method for determination of micro¬quantities of vanadium the relative error of which is not more than 8–10%.

Influence of age and gender on the levels of cytogenetic disturbances in exfoliated oral mucous cells of healthy non-smoking Armenians by means of micronucleus assay is analysed. It is revealed the increase of the micronuclei levels of women (compared with men) and of older persons (compared with younger ones).