Vol. 40 No. 1 (209) (2006)

In the present article some modem techniques, which allow qualitative and quantitative analyses in nm scale of solid surfaces with a thickness equal to some atomic layers are described. For an analysis of thin layers of surfaces classical methods are not applicable, because a very small quantity of material is used ($\leq$ 10^-13 g/cm²) and the signal coming from surface layer is covered by a large signal of the bulk. Described methods are widely applied in the study of polymers' surfaces, specially, in analyses of biomaterial surfaces.

It is shown that the H₂O₂ +HOO^- reaction rate is more in inverse emulsions stabilized by lecitin (Le) than in water solutions. In this case the reaction occurs in micelles nucleus. One of the Le pecularities is that it is a zwitterion type surfactant. In its molecule there are (-) and (+) charged «heads» which act on the cited reaction rate. This conclusion is verified adding different quantities of anionic (sodium dodecyl sulfate) and cationic (dodecyltrimethylammonium chloride) surfactants isolating in turn both charged heads with different extents and studying the reaction rate dependence on the isolation degree. The reaction rate is determined measuring the volume of the evoluated oxygen. According to obtained kinetic data both «heads» are active, but the (+) charged head is more active.

Absorbed IR spectrums of vitamin E in presence of dimethyl-, diethylsulphoxides in CCl₄ have been investigated. It has been shown that complex-formation of vitamin E with sulphoxides is the result of formation of hydrogen bond between hydrogen of vitamin E hydroxyl group and oxygen of the sulphoxides S=0 group. It have been established the equilibrium constants (K) of vitamin E complexes with dimethylsulphoxide (K=13.2 L/mol) and diethylsulphoxide (K=18.2 L/mol) at 23℃.

The complex formation of chrome(III) with sodium salicylate in water (aqua) solution is studied by spectrophotometric method. It is established that a step-by-step complex formation takes place. The contents and constants of instability of the formed complexes are defined.

The interaction of palladium(II) with allylthiourea (ATM) has been studied by means of amperometry method. The molar ratio of the reacting components is [Pd(II)]:[ATM]=1:4. Conditions for carrying electrode processes on hydrochloric and sulfuric acid backgrounds have been studied. Palladium(II) concentrations submitting to the amperometry main law have been determined.

The method of synthesis of a new modified chiral auxiliary (S)-N-(2-benzoylphenyl)-1-(4-benzyloxybenzyl) pyrrolidine-2-carboxamide and plain- square Ni(II) complexes of ShifFs basis with glycine and alanine has been developed. The synthesis of chiral auxiliary has been carried out in the presense of CH₂Cl₂ at 0°C via condensation of 2-aminobenzophenone with p-benzyl-oxybenzylproline, through intermediate formation of chloranhydride in the presense of PCl₅. On the basis of the synthesized chiral auxiliary plain-square Ni(II) complexes of it’s Shiff s base of glycine and alanine has been synthesized. The synthesis was carried out in CH₃OH in the presense of strong base (KOH) at 55–60°C. As expected during the synthesis of the alanine complex a mixture of diastereoisomeric complexes S,S/S,R in a ratio 98:2 has been deriveted.

The contents of chromatin phospholipid and nuclear membrane of germination cereal seeds have been investigated. It is shown, that the investigated nuclear sub fractions of cereal seedlings – nuclear membrane and chromatin differ in content and composition of phospholipids. At treatment of seeds with gibberellin (GA₃) has occurred the redistribution of phospholipids in content and composition. It is shown that specimens of nuclear membranes and chromatin of germinating cereal seeds differ both in content and composition of phospholipids. In studied nuclear sub fractions the sensibility to influence of factors of different nature show different classes of phospholipids.

Induction of somatic recessive mutations and micronuclei by solutions of salts of heavy metals CrCl₃, Pb(NO₃)₂, ZnSO₄, NiCl₂, CuCl₂ and chrome oxide Cr₃O in test-systems of Tradescantia (clone 02) is investigated. It is shown, that ions of investigated heavy metals are genotoxic. They induce somatic point mutations and clastogen effect in sporogenic cells of Tradescantia.

Dynamic changes of cortex various areas activity were investigated with the purpose of revealing the cortical structures responsible for realization of the aggressive game by examinees – teenagers with different degree of initial aggression. The increase of component N₂₀₀ amplitude in frontal areas and decrease of amplitude in temporal areas after presented game is observed at teenagers with a high level of initial aggression. At the same time, after presented game it is shown the decrease of component N₂₀₀ amplitude in frontal areas and increase of amplitude in temporal areas at teenagers with a low level of initial aggression.

With the use of new approaches and modem experimental methods of research the complex study of parameters background neuronal activity (BNA) of medial vestibular nucleus (MVN) in norm and various terms after unilateral labyrinthectomy. It is shown, that in 2 days after delabyrinthation the dynamic balance existing between vestibular nuclei of both parts in norm, is broken. The comparative analysis impulse activity in 7days unilateral labyrinthectomy has revealed an identical level neurodynamic activity kontra- and ipsilateral MVN.

The interaction of analginum anion with dye of diaminoksantene raw rhodamine C has been studied. Formed ionic associate could be extracted once trough (R=0.975) by dichlorethane-amilacetate (1:4) binary mixture from solutions pH 1 to 1.0 N HCl and rhodamine C is 3.7·10^-4–4.6·10^-4M. The range of determined concentration of analginum is 2.4·10^-5–l.44·10^-4 M. The molar coefficient of extraction $\epsilon_{565}$ = 4.2·10⁴ L·mol^-1·cm^-1. The method was used for determining small amounts of analginum in spazmalgonum, baralginum and pentalginum.

The antitumour drug cis-diamminedichloroplatinum(ll) (cis-DDP) form covalent adduct with DNA both in vivo and in vitro. In the present work, UV-spectroscopic helix-coil transition method is used to analyze the changes of the high-molecular DNA double helix occur due to formation of complexes with cis-DDP at the concentration of cis-DDP 3·10^-5<r_b<3·10^-3, where r_b=C_cis-DDP/C_{bound nucl.}.

Elevated homocysteine level in blood is known as independent risk factor for ischemic stroke. One of the most common causes of hyperhomocysteinaemia is methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation. 43 patients with last month ischemic stroke incident were investigated and compared with 39 healthy (controls). Total plasma homocysteine levels was significantly high in cases versus controls (18.94±1.49 and 9.77±0.86 (μmol/L), p<0.001). Mutations frequency concurs with available investigations results. Tendency of homocysteine level elevation connected with heterozygous and homozygous mutation of MTHFR gene was found.