THE CYTOTOXIC PROPERTIES OF $ALCHEMILLA~SMIRNOVII$ EXTRACT ON THE HELA CANCER CELLS VIA DOWNREGULATION OF ARGINASE ACTIVITY

Authors

  • Svetlana M. Hovhannisyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia
  • Edita E․ Nadiryan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia https://orcid.org/0000-0002-4998-6419
  • Mery V. Qocharyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia
  • Gayane H. Petrosyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia
  • Gohar Sevoyan L.A. Orbeli Institute of Physiology NAS RA, Armenia
  • Zaruhi I. Karabekian L.A. Orbeli Institute of Physiology NAS RA, Armenia https://orcid.org/0000-0002-8693-7288
  • Hayarpi G. Juvrushyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia https://orcid.org/0000-0002-6941-8283
  • Mikayel M․ Ginovyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia https://orcid.org/0000-0002-8585-5539
  • Nikolay V. Avtandilyan Chair of Biochemistry, Microbiology and Biotechnology, YSU, Armenia; Research Institute of Biology, Laboratory of Basic and Pathological Biochemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2023.57.3.238

Keywords:

Alchemilla smirnovii, HeLa, anticancer effect, 5-fluorouracil, arginase

Abstract

Plants of the genus Alchemilla are well known for their pharmacological effects. Alchemilla smirnovii Juz., also known as lady's mantle possessed high growth-inhibiting properties against cancer cells according to earlier reports. We aimed to explore the anticancer modulatory effect of A. smirnovii extract toward 5-fluorouracil and elucidate the possible mechanism of its cytotoxic action in the HeLa cancer cell model. Synergistic interactions of plant extract with the chemotherapeutic agent were assessed by MTT assay. Based on obtained data the ethanol extract of A. smirnovii exhibits synergistic effects, enhancing the growth-inhibiting properties of 5-fluorouracil when used together. Additionally, the extract leads to a reduction in arginase activity and an elevation in the level of MDA․ This implies that A. smirnovii induces oxidative stress, and can potentially cause apoptosis in cancer cells.

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Published

2023-12-18

How to Cite

Hovhannisyan, Svetlana M., Edita E․ Nadiryan, Mery V. Qocharyan, Gayane H. Petrosyan, Gohar Sevoyan, Zaruhi I. Karabekian, Hayarpi G. Juvrushyan, Mikayel M․ Ginovyan, and Nikolay V. Avtandilyan. 2023. “THE CYTOTOXIC PROPERTIES OF $ALCHEMILLA~SMIRNOVII$ EXTRACT ON THE HELA CANCER CELLS VIA DOWNREGULATION OF ARGINASE ACTIVITY”. Proceedings of the YSU B: Chemical and Biological Sciences 57 (3 (262):238-47. https://doi.org/10.46991/PYSU:B/2023.57.3.238.

Issue

Vol. 57 No. 3 (262) (2023)

Section

Biology

License

Copyright (c) 2023 Proceedings of the YSU

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