Vol. 39 No. 2 (207) (2005)

The reaction between mono- and dimethylolurea with chlorydes of metals Co(II), Ni(II) has been explored. The synthesized connections were investigated by the UR-, NMR-spectrum methods of analysis of elements, and also their magnetic susceptibility is measured.

Interaction of palladium(II) with N,N-diphenyl- and N,N'-diphenylthioureas has been studied by means of amperometric titration method. Passing of the reactions has been recorded with Pd(II):R=l:2 and Pd(II):R=l:4 molar ratio between the reacted components. Determination of the palladium(II) is possible within wide concentration range of the sulphuric and hydrochloric acids. Statistical mathematical treatment of the experimental data has been carried out. Technique for amperometric determination of the palladium using the above-mentioned reagents has been elaborated and applied for its determination in the palladium-plating electrolyte.

4-Hydroxy-3-(2-chlorprop-2-enyl)-2-methylquinolines were synthesized by O-allylation of corresponding 4-hydroxy-2-methylquinolines and further rearrangement of resulting 4-(2-chlorprop-2-enyloxy)-2-methylquinolines. As a result of following reactions, they were converted to 4-amino-3-(2'-methylindolyl-3')-2-methylquinolines.

A synthesis of benzotriazole derivatives by interaction of benzotriazole sodic derivative with l,2-di(chloromethyl)4,5-dimethylbenzene, l,5-di(chloromethyl)2,4-dimethylbenzene, l,4-di(chloromethyl)2,5-dimethylbenzene, ethyl chloroacetat, epichlorohydrin, ethylene chlorohydrin and 2-aminobenzoyl chloride have been realized.

Numerical aberrations of 19 patients with acute myeloid leukemia have been studied by the technique of Fluorescence in situ hybridization (FISH) in interphase cells. The centromere specific DNA probes for chromosomes 8, 10, 11, 7, X and Y were prepared in the Department of Genetics and Cytology of YSU. We revealed different numerical aberrations of tested chromosomes (not less than 3%) at 10 patients. Near-tetraploidy was found at one of them. FISH method in interphase cells allowed to reveal cytogenetic abnormalities at patients with AML, as the conventional cytogenetic analysis is inaccessible, and screening application is recommended by us.

The fluorescence spectrum of bovine liver arginase excited at 257 nm is similar to that of excited at 291 nm. The alteration of pH values, hydroxylamine treatment and prolonged dialysis leads to the changes in the ratio of fluorescence maximal intensities at mentioned excitation wavelengths (I₂₅₇^max / I₂₉₇^max), which reflects, probably the alteration of microenvironment of aromatic amino acids residues in arginase. The comparative study of the fluorescence spectra of protein solutions at the excitation wavelength 257 and 291 nm can be used as a sensitive method to receive new information about small conformational changes of protein molecules.

An example of five species of wine yeast the possibility of maximum extraction of amino acids is shown in multiple treatment by hot ethanol, permissive to extract majority of aliphatic, dicarbonic, dibasic and aromatic amino acids.

Callus cultures of alkanet (Alkanna orientalis) of leaf, root and stalk origin able to synthesize naphthoquinones are obtained. Increase of cytokinin content in the nutrient medium promotes to synthesize pigments' quantity rising. In callus culture of root origin by means of HPLC the shikonin was identified.

In the case of enduring influence of vibration, blood indexes have certain peculiarities. In the initial stage the number of peripheral blood indexes increases, which is important for securing the adaptable protective mechanisms of the organism, and in the case of prolonged influence, an enduring reduction of erythrocytes and hemoglobin, leucopoenia, marked limpho-, eozinopenia are observed, which is an evidence of prolipherative pressure of hemopoes.

On the territory of «Sevan» National Park with buffer zone was found 113 species of edible basidiomycetes. The revealed mushrooms belong to 6 ecological groups. Seasonal dynamics of fructification is investigated too, the most widespread species are given.

An interaction between rhodium(III) chlorice acidocompiex and thiazine raw organic basic dye dimethylthionine was studied. Compound forming in the system is extracted by dichloroethane–carbon tetrachloride (2.5+2.5 mL) binary mixture. Maximal light absorbance (extinction) for extracts of forming compound as well as for blind extracts is observed at 620 nm wavelenght. At the optimal acidity conditions (pH 1.0 by hydrochloric acid) rhodium(III) is extracted almost quantitatively (R=94%) to the organic solvent phase by means of single extraction during 1 minute. The shaking optimal quantity of dimethylthionine is secured by the addition of 1.0–1.4 mL of 0.05% solution of the dye. Dichlorethane–carbon tetrachloride–aceton extracts of coloured compound submit to the photometry main law in the range of rhodium(III) content in aqueous phase 5.48–43.8 mkg/10 mL. The molar coefficient of extinction is  ε₆₂₀=1.3–10⁴ L· mol^-1·cm^-1. C_mn=0.03 mkg Rh/mL. The influence of a series of interfering and accompanying elements on the determination of the rhodium(III) has been studied. An extraction-absorptiometric method for determination of rhodium by dimethylthionine has been elaborated.

As a continuation of our investigations in the field of creation of potential (3-adrenoblokers, the method for realization of interaction of l,2-epoxy-3-naphthoxy-propane with some α- and β-aminoacids has been developed and proposed. In particular the aminoacids don’t react with epoxides, as they are in the form of internal salts. For the purpose of realization the interaction with some α- and β-aminoacids, they were transformed into their sodium salt, which easily interact with an epoxides according to Krasusky’s rule. As a result N-(naphthoxy-2-hydroxypropil) aminoacids were obtained.

Localization of genes incompatibility (inc) on the DNA R906 plasmide map is fulfilled through revealation of P-1 specific incompatibility by various deletion and recombination derivatives of R906 plasmide. It has been carried out by the analyses of segregation kinetics in the case of combining the above mentioned derivatives in a cell as a result of their reciprocal transformation or conjugation with Inc P-1 plasmides R751 and R702. It has become possible to localize inc genes on the DNA sector with 24.6–25.7 kb coordinates comparing the received data with the restriction maps of the derivatives of R906 plasmide.

In conditions of acute experiment on rats anaesthetized by nembutal (40 mg/kg, interperitoniaily) the registration and analysis of background impulse activity of supraoptic nucleus' neurons of rats’ hypothalamus in norm and after electromagnetic irradiation of extremely high frequency on organism were carried out. Changes of neurons background impulse activity mainly related to internal structure of recorded impulse flows were revealed. It was shown that significant shifts generally are observed in the character of dynamics of neuronal current flows. Statistically significant changes of average frequency of neuronal impulsation were not observed.

The correlated interrelations between the sexual belonging, non-verbal IQ parameters, creativity and efficiency of solving the spatial-shaped task of mazemodel type and the neurophysiologic bases of gender differences in efficiency of spatial orientation were studied. Authentic dominating influence of sex and non-verbal IQ size on efficiency of maze-model task performance is shown. Gender differences of mechanisms of maintenance of the spatial tasks decision are revealed: connected activation of occipital areas and parieta) and frontal areas is found out in men, against tested women to whom activation of only occipital area suffices.