LACTATE DEHYDROGENASE ISOENZYMES PATTERN AND MORPHOGENETIC POTENTIALS OF THE CALLUS CULTURES

Authors

  • A. V. Nerkararian Chair of Biophysics, YSU, Armenia
  • H. R. Vardapetian Chair of Biophysics, YSU, Armenia
  • A. B. Kirakosian Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2001.35.3.095

Abstract

Lactate dehydrogenase (LDH) total activity and isoenzymes pattern were determined in organs of germs and cell cultures of wheat, amaranth, as well as in their somatic hybrid. The LDH isoenzymes relative activities were calculated. It was shown that the application of common methods of assay of the individual isoenzymes activities and quantitative ratio does not permit to reveal the true value of these parameters. It was shown that the quantitative ratios of separate subunits (H/M) reflect the morphogenetic potentials of the individual and hybrid callus cultures. The possibility of the application of isoenzymes quantitative and qualitative pattern as express test for cell cultures morphogenetic potentials and regeneration abilities determination was discussed.

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Published

2001-10-31

Issue

Vol. 35 No. 3 (196) (2001)

Section

Biology

License

Copyright (c) 2001 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“LACTATE DEHYDROGENASE ISOENZYMES PATTERN AND MORPHOGENETIC POTENTIALS OF THE CALLUS CULTURES”. 2001. Proceedings of the YSU B: Chemical and Biological Sciences 35 (3 (196): 95-101. https://doi.org/10.46991/PYSUB.2001.35.3.095.