INVESTIGATION OF DNA THERMAL DENATURATION AT THE PRESENCE OF DIALKYLSULFOXIDES BY FLUORESCENCE SPECTROSCOPY

Authors

  • M. G. Aznauryan Chair of Physical and Colloids Chemistry, YSU, Armenia
  • A. G. Khachatryan Chair of Physical and Colloids Chemistry, YSU, Armenia
  • Sh. A. Markaryan Chair of Physical and Colloids Chemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2010.44.1.008

Keywords:

thermal denaturation of DNA, dialkylsulfoxides, ethidium bromide, fluoresence spectroscopy

Abstract

The effect of dialkylsulfoxides (DASO) on the thermal denaturation of DNA was studied by fluoresence spectroscopy. As a fluorescence probe the ethidium bromide was used. It is revealed, that DASO shows expressed denaturing ability. In series of DMSO–DESO–DPSO with increasing of hydrocarbonic chain length of sulfoxides their denaturing ability increases, which is caused by the increase of direct hydrophobic interactions between DNA–DASO.

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Published

2010-02-24

How to Cite

Aznauryan, M. G., A. G. Khachatryan, and Sh. A. Markaryan. 2010. “INVESTIGATION OF DNA THERMAL DENATURATION AT THE PRESENCE OF DIALKYLSULFOXIDES BY FLUORESCENCE SPECTROSCOPY”. Proceedings of the YSU B: Chemical and Biological Sciences 44 (1 (221):8-11. https://doi.org/10.46991/PYSU:B/2010.44.1.008.

Issue

Vol. 44 No. 1 (221) (2010)

Section

Chemistry

License

Copyright (c) 2010 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.