IMPROVEMENT OF PURIFICATION PROCEDURE OF D-AMINO ACID OXIDASE FROM $CANDIDA~GUILLIERMONDII$

Authors

  • G.K. Gevorgyan Chair of Microbiology, Plants and Microbes Biotechnology, YSU, Armenia
  • M.A. Davtyan Chair of Microbiology, Plants and Microbes Biotechnology, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2012.46.2.041

Keywords:

$Candida~guilliermondii$, D-amino acid oxidase, DL-alanine, chromatofocusing, hydrophobic chromatography

Abstract

The purification procedure of D-amino acid oxidase from Candida guilliermondii was improved. An initial induction of the enzyme was performed by adding DL-alanine to cultivation medium composition. By means of twice chromatofocusing and without the step of hydrophobic chromatography it was available to obtain purified preparation of D-amino acid oxidase with higher yield.

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Published

2012-05-10

Issue

Vol. 46 No. 2 (228) (2012)

Section

Biology

License

Copyright (c) 2012 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“IMPROVEMENT OF PURIFICATION PROCEDURE OF D-AMINO ACID OXIDASE FROM $CANDIDA~GUILLIERMONDII$”. 2012. Proceedings of the YSU B: Chemical and Biological Sciences 46 (2 (228): 41-45. https://doi.org/10.46991/PYSUB.2012.46.2.041.