NEW MODE OF ENDOINULASE (EC 3.2.1.7) PURIFICATION

Authors

  • G.Ye. Baghdasaryan Chair of Biology and Chemistry, Goris State University, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2014.48.3.058

Keywords:

inulin, endoinulase, fructose, glucose

Abstract

At the first time the mode of endoinulase purification from dapple has been proposed. Endoinulase was released from Taraxacum officinale root cells, solved in water, settled at pH 6.2, then the sediment was treated by buthanol. The purification was obtained by gel-filtration method applying organic solvents (buthanol and acetone) as well as a column containing G-150 Sephadex (with 2.0×120 cm2 sizes). The enzyme was purified 71.3 times, its molecular mass was 176 kDa.

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Published

2014-11-14

How to Cite

Baghdasaryan, G.Ye. 2014. “NEW MODE OF ENDOINULASE (EC 3.2.1.7) PURIFICATION”. Proceedings of the YSU B: Chemical and Biological Sciences 48 (3 (235):58-61. https://doi.org/10.46991/PYSU:B/2014.48.3.058.

Issue

Vol. 48 No. 3 (235) (2014)

Section

Short Communications

License

Copyright (c) 2014 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.