STUDY OF INTERACTION OF HOECHST 33258 AND DNA IN WATER–DMSO MIXED SOLVENTS

Authors

  • K.Yu. Amirbekyan Chair of Physical and Colloids Chemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2016.50.1.010

Keywords:

Hoechst 33258, Calf Thymus DNA, UV/vis spectroscopy, thermal melting, dimethyl sulfoxide, molecular interactions

Abstract

Interaction of well-known DNA minor groove binder Hoechst 33258 and Calf Thymus DNA without and in the presence of increasing amount of dimethyl sulfoxide (DMSO) was studied using UV-vis spectroscopy methods. Thermal melting results show that the melting temperature of Hoechst–DNA complex decreases while increasing of DMSO content. It was shown that DNA is more stable at low DMSO content (5% v/v) rather, than at high concentrations (10–20%).

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Published

2016-03-31

How to Cite

Amirbekyan, K.Yu. 2016. “STUDY OF INTERACTION OF HOECHST 33258 AND DNA IN WATER–DMSO MIXED SOLVENTS”. Proceedings of the YSU B: Chemical and Biological Sciences 50 (1 (239):10-13. https://doi.org/10.46991/PYSU:B/2016.50.1.010.

Issue

Section

Chemistry