CELL-FREE MITOCHONDRIAL DNA IN DOXORUBICIN-TREATED KCL22 AND K562 CELL LINES

Authors

  • Tigran A. Harutyunyan Chair of Genetics and Cytology, YSU, Armenia; Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0000-0002-1613-1019
  • Anzhela A. Sargsyan Chair of Genetics and Cytology, YSU, Armenia; Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0000-0003-2720-0355
  • Lily A. Kalashyan Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0009-0004-0882-4984
  • Hovhannes A. Igityan Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0000-0003-4923-5583
  • Rouben M. Aroutiounian Chair of Genetics and Cytology, YSU, Armenia; Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0000-0003-1020-9513
  • Galina G. Hovhannisyan Chair of Genetics and Cytology, YSU, Armenia; Laboratory of General and Molecular Genetics, Research Institute of Biology, YSU, Armenia https://orcid.org/0000-0002-7450-1508

DOI:

https://doi.org/10.46991/PYSU:B.2024.58.1.009

Keywords:

doxorubicin, cell-free mtDNA, PCR, gel electrophoresis, cytotoxicity, genotoxicity, KCL22 and K562 cells

Abstract

Cell-free mitochondrial DNA (cf-mtDNA) is widely used as a biomarker of pathological processes, but its application for assessing the effects of environmental factors is limited. For the first time, cf-mtDNA was identified in the KCL22 cell culture treated with doxorubicin, which is known for its ability to disrupt mitochondrial functions. The results obtained indicate the effectiveness of using cf-mtDNA as a biomarker of genotoxic and cytotoxic effects.

Published

2024-04-24

How to Cite

Harutyunyan, Tigran A., Anzhela A. Sargsyan, Lily A. Kalashyan, Hovhannes A. Igityan, Rouben M. Aroutiounian, and Galina G. Hovhannisyan. 2024. “CELL-FREE MITOCHONDRIAL DNA IN DOXORUBICIN-TREATED KCL22 AND K562 CELL LINES”. Proceedings of the YSU B: Chemical and Biological Sciences 58 (1 (263):9-16. https://doi.org/10.46991/PYSU:B.2024.58.1.009.

Issue

Vol. 58 No. 1 (263) (2024)

Section

Biology

License

Copyright (c) 2024 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.