THE INVESTIGATION OF THE INFLUENCE OF pH AND UREA ON THEFLUORESCENCE OF ARGINASE SOLUTIONS

Authors

  • M. I. Gevorkian Chair of Biochemistry, YSU, Armenia
  • M. A. Davtian The Laboratory of Comparative and Evolutionary Biochemistry, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2002.36.3.085

Abstract

The investigation of the influence of pH on the fluorescence spectra of bovine liver arginase in solutions has shown that in the region of pH 6.5–9.8, where the enzyme keeps the activity, small conformational changes take place. At the extremal pH values (2.8 and 10.5) and in the presence of high concentrations of urea the intensivity of fluorescence decreases and maximum spectra are shifted. The data suggested that polypeptide chain of arginase is damaged in 8 M urea solutions without any cleavage of disulfide bonds.

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Published

2002-09-20

Issue

Vol. 36 No. 3 (199) (2002)

Section

Biology

License

Copyright (c) 2002 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“THE INVESTIGATION OF THE INFLUENCE OF PH AND UREA ON THEFLUORESCENCE OF ARGINASE SOLUTIONS”. 2002. Proceedings of the YSU B: Chemical and Biological Sciences 36 (3 (199): 85-90. https://doi.org/10.46991/PYSUB.2002.36.3.085.