DEPENDENCE OF EtBR AND Hoechst 33258 BINDING WITH DNA ON IONIC STRENGTH OF SOLUTION

Authors

  • K. V. Pirumyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2008.42.2.100

Keywords:

melting of DNA, ethidium bromide, Hoechst 33258, ionic strength

Abstract

Melting of DNA and it complexes with ethidium bromide (EtBr) and Hoechst 33258 has been carried out at μ=2·10-3 and μ=2·10-2 M Na+ ionic strength of solution. It is revealed, that specificity of Hoechst 33258 to the AT sequences is observed at μ=2·10-2 M Na+ ionic strength, whereas decreasing of ionic strength in one order leads to disappearance of specificity. It is shown that EtBr doesn’t show specificity to the obtained sequences in the same conditions.

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Published

2023-09-22

How to Cite

Pirumyan, K. V. 2023. “DEPENDENCE OF EtBR AND Hoechst 33258 BINDING WITH DNA ON IONIC STRENGTH OF SOLUTION”. Proceedings of the YSU B: Chemical and Biological Sciences 42 (2 (216):100-103. https://doi.org/10.46991/PYSU:B/2008.42.2.100.

Issue

Vol. 42 No. 2 (216) (2008)

Section

Biology

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Copyright (c) 2023 Proceedings of the YSU

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