RESEARCHE OF Hoechst 33258 BINDING WITH DNA

Authors

  • A. P. Antonyan Chair of Biophysics, YSU, Armenia
  • K. V. Pirumyan Chair of Biophysics, YSU, Armenia
  • M. S. Mickaelyan Chair of Biophysics, YSU, Armenia
  • P. O. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2007.41.3.112

Keywords:

fluorescent and absorption methods, Hoechst 33258, binding, type of interaction

Abstract

Research of interaction of Hoechst 33258 with DNA, binding through a minor groove of DNA, by spectroscopic (fluorescent and absorption) methods is carried out. It is revealed, that this ligand with DNA binds by two modes – strong and weak. The strong type of interaction Hoechst 33258 with DNA is detected by both fluorescent and absorption methods, while the weak type is observed only by the absorption method. On the basis of the analysis of the spectroscopic data values of parameters of linkage - K and n are calculated.

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Published

2007-10-12

How to Cite

Antonyan, A. P., K. V. Pirumyan, M. S. Mickaelyan, and P. O. Vardevanyan. 2007. “RESEARCHE OF Hoechst 33258 BINDING WITH DNA”. Proceedings of the YSU B: Chemical and Biological Sciences 41 (3 (214):112-17. https://doi.org/10.46991/PYSU:B/2007.41.3.112.

Issue

Vol. 41 No. 3 (214) (2007)

Section

Biology

License

Copyright (c) 2007 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.