FORMATION OF SEMI-INTERCALATION COMPLEXES AT EtBr INTERACTION WITH DNA

Authors

  • A. P. Antonyan Chair of Biophysics, YSU, Armenia
  • H. G. Davtyan Chair of Biophysics, YSU, Armenia
  • P. O. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2003.37.3.097

Keywords:

Etidium Bromide, ds-DNA, binding constant, number of the bases

Abstract

The features of EtBr complex-formation with DNA are investigated. It is shown, that the chromophore ring of ligand can be inserted into a plane between the next bases in one of strands of double-stranded (ds-DNA) (semi-intercalation complex). The binding curves by semi-intercalative interaction of EtBr with DNA are obtained and the parameters of interaction binding constant (K) and the number of the bases (n), corresponding to one binding site, are determined. It is revealed, that EtBr may bind with completely denatured, single-stranded (ss) DNA, thus, the mechanism of interaction of ligand with ss-DNA also has semi- intercalative character, and the binding parameters  K and n coincide with those received at semi-intercalative interaction of EtBr with ds-DNA.

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Published

2003-10-09

Issue

Vol. 37 No. 3 (202) (2003)

Section

Biology

License

Copyright (c) 2003 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“FORMATION OF SEMI-INTERCALATION COMPLEXES AT EtBr INTERACTION WITH DNA”. 2003. Proceedings of the YSU B: Chemical and Biological Sciences 37 (3 (202): 97-102. https://doi.org/10.46991/PYSUB.2003.37.3.097.