CELL VIABILITY AND DNA DAMAGE IN MRC5 AND HeLa CELL LINES AFTER HISTONE H1 KNOCKOUT BY CRISPR-Cas9 GENOME EDITING TECHNOLOGY

Authors

  • T.A. Harutyunyan Chair of Genetics and Cytology, YSU, Armenia
  • N.S. Babayan Chair of Genetics and Cytology YSU, Armenia, Institute of Molecular Biology, NAS RA
  • R.M. Aroutiounian Chair of Genetics and Cytology YSU, Armenia
  • G.G. Hovhannisyan Chair of Genetics and Cytology, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2016.50.3.064

Keywords:

CRISPR-Cas9, comet assay, HeLa, HIST1H1B gene, MRC5

Abstract

Chromatin research mainly focused on the core histones, whereas the role of H1.5 linker histone is poorly understood. Today CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats associated protein 9) genome editing technology provides an opportunity to analyze functions of different genes introducing targeted loss-of function mutations. Here we demonstrate the role of histone H 1.5 in cell viability and genome integrity in HeLa and MRC5 cells using trypan blue exclusion test and single-cell gel electrophoresis (comet) assay.

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Published

2016-11-21

Issue

Vol. 50 No. 3 (241) (2016)

Section

Short Communications

License

Copyright (c) 2016 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“CELL VIABILITY AND DNA DAMAGE IN MRC5 AND HeLa CELL LINES AFTER HISTONE H1 KNOCKOUT BY CRISPR-Cas9 GENOME EDITING TECHNOLOGY”. 2016. Proceedings of the YSU B: Chemical and Biological Sciences 50 (3 (241): 64-66. https://doi.org/10.46991/PYSUB.2016.50.3.064.

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