ERROR IN DETERMINATION OF BINDING THERMODYNAMIC PARAMETERS APPEARED DUE TO ADSORPTION LINEAR ISOTHERM DESCRIPTION

Authors

  • S.N. Hakobyan Department of Machine Science, National Polytechnic University of Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2015.49.2.040

Keywords:

synthesized polyribonucleotide, ethidium bromide, description modes of binding isotherm, linear mode, thermodynamic parameters

Abstract

In the present work the description of absorption spectra of ethidium bromide complexes with synthesized polyribonucleotide by several modes has been carried out. The binding constant value and the values of changes of thermodynamic parameters were determined. It was shown that at the description by linear binding isotherm the value of enthalpy and the number of nucleotides corresponding to one ligand molecule at interaction saturation are approximately the same as real ones, but the values of the binding constant, Gibbs free energy change and entropy change differ from real values.

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Published

2015-07-10

How to Cite

Hakobyan, S.N. 2015. “ERROR IN DETERMINATION OF BINDING THERMODYNAMIC PARAMETERS APPEARED DUE TO ADSORPTION LINEAR ISOTHERM DESCRIPTION”. Proceedings of the YSU B: Chemical and Biological Sciences 49 (2 (237):40-44. https://doi.org/10.46991/PYSU:B/2015.49.2.040.

Issue

Vol. 49 No. 2 (237) (2015)

Section

Biology

License

Copyright (c) 2015 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.