DETERMINATION ERROR OF NUCLEIC ACID–LIGAND BINDING THERMODYNAMIC PARAMETERS CONDITIONED BY DETERMINATION ERROR OF PHYSICAL CHARACTERISTICS OF COMPLETELY BOUND STATE OF LIGAND

Authors

  • S.N. Hakobyan Department of Machine Science, National Polytechnic University of Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2018.52.1.063

Keywords:

poly(G)·poly(C), ethidium bromide, mitoxantrone, binding parameters, determination error

Abstract

To construct the binding isotherms of nucleic acid–ligand it is necessary to determine physical characteristics of completely bound state of ligand (Ab) experimentally. It is often impossible to determine Ab experimentally, due to which as Ab the value of the parameter is accepted, which almost does not change in the experiment error framework. Calculations show that in this case the binding constant determination error does not exceed 10%.

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Published

2018-03-15

How to Cite

Hakobyan, S.N. 2018. “DETERMINATION ERROR OF NUCLEIC ACID–LIGAND BINDING THERMODYNAMIC PARAMETERS CONDITIONED BY DETERMINATION ERROR OF PHYSICAL CHARACTERISTICS OF COMPLETELY BOUND STATE OF LIGAND”. Proceedings of the YSU B: Chemical and Biological Sciences 52 (1 (245):63-66. https://doi.org/10.46991/PYSU:B/2018.52.1.063.

Issue

Vol. 52 No. 1 (245) (2018)

Section

Biology

License

Copyright (c) 2018 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.