MELTING PARAMETERS OF DNA COMPLEXES WITH SEVERAL INTERCALATORS

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.A. Parsadanyan Chair of Biophysics, YSU, Armenia
  • V.G. Sahakyan Chair of Biophysics, YSU, Armenia
  • P.G. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2017.51.1.027

Keywords:

DNA, acridine orange, ethidium bromide, intercalation, methylene blue, melting curve

Abstract

Comparative study of DNA complexes with acridine orange (AO), ethidium bromide (EtBr) and methylene blue (MB) has been carried out at 0.02 M ionic strength of solution in 0 < r $\leq$ 0.33, r = [ligand] / [DNA] range. The values of changes of melting parameters ($\delta(1/T_m)$ and $\delta(\Delta T/T_m^ 2))$ of these complexes were determined. From the obtained data it was revealed that the dependence of $\delta (1/T_m)$ enhances in the whole interval of change of r for three ligands, while the dependence of $\delta(\Delta T/T_m^ 2)$ rises at relatively low values of r and comes out to plateau in the case of AO and MB; in the case of EtBr this dependence takes bell-like form.

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Published

2017-03-30

How to Cite

Antonyan, A.P., M.A. Parsadanyan, V.G. Sahakyan, and P.G. Vardevanyan. 2017. “MELTING PARAMETERS OF DNA COMPLEXES WITH SEVERAL INTERCALATORS”. Proceedings of the YSU B: Chemical and Biological Sciences 51 (1 (242):27-30. https://doi.org/10.46991/PYSU:B/2017.51.1.027.

Issue

Vol. 51 No. 1 (242) (2017)

Section

Biology

License

Copyright (c) 2017 Proceedings of the YSU

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