STUDY OF STRUCTURAL TRANSITIONS OF COMPLEXES OF DIFFERENT LIGANDS WITH DNA AND BSA

Authors

  • Poghos H. Vardevanyan Chair of Biophysics, YSU, Armenia
  • Nara R. Petrosyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2022.56.1.049

Keywords:

biomacromolecule, ligand, binding modes, UV-denaturation method, transition temperature

Abstract

Structural transitions of DNA and bovine serum albumin (BSA) and their complexes with methylene blue (MB) and Hoechst 33258 (H33258) by UV-denaturation method have been studied. The obtained results revel that MB stabilizes both protein dimensional structure and DNA double-stranded structure, while H33258 stabilizes double-stranded structure of DNA and destabilizes protein structure. It was also revealed that the stabilizing effect of MB on native structure of DNA is more pronounced at relatively low ligand concentrations, than on protein.

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Published

2022-05-03

How to Cite

Vardevanyan, Poghos H., and Nara R. Petrosyan. 2022. “STUDY OF STRUCTURAL TRANSITIONS OF COMPLEXES OF DIFFERENT LIGANDS WITH DNA AND BSA”. Proceedings of the YSU B: Chemical and Biological Sciences 56 (1 (257):49-55. https://doi.org/10.46991/PYSU:B/2022.56.1.049.

Issue

Vol. 56 No. 1 (257) (2022)

Section

Biology

License

Copyright (c) 2022 Proceedings of the YSU

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