SPECTROPHOTOMETRIC STUDY OF COMPLEXES OF SOME INTERCALATORS WITH DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS

Authors

  • Ara P. Antonyan Chair of Biophysics, YSU, Armenia
  • Zvart O. Movsisyan Chair of Biophysics, YSU, Armenia
  • Anzhela H. Karapetyan Chair of Biophysics, YSU, Armenia
  • Poghos H. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2023.57.3.214

Keywords:

DNA, synthetic polynucleotides, classical and non-classical intercalators, absorption spectra, binding mode

Abstract

Interaction of ethidium bromide (EtBr), acridine orange (AO) and non-intercalator methylene blue (MB) with DNA as well as interaction of EtBr and MB with single-stranded synthetic polynucleotides poly(rA) and poly(rU) have been studied. It was revealed that in absorption spectra of EtBr and MB with DNA or single-stranded polynucleotides a real- or pseudo-isosbestic point appears. Particularly, in absorption spectra of the complexes of classical intercalator EtBr with DNA the real isosbestic point is formed, but in the case of non-classical intercalator MB a pseudo-isosbestic point is formed, while in the absorption spectra of their complexes with single-stranded polynucleotides a pseudo-isosbestic point is formed. It was found out that for another classical intercalator AO there is no a real or pseudo-isosbestic point.

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Published

2023-10-05

Issue

Vol. 57 No. 3 (262) (2023)

Section

Biology

License

Copyright (c) 2023 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“SPECTROPHOTOMETRIC STUDY OF COMPLEXES OF SOME INTERCALATORS WITH DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS”. 2023. Proceedings of the YSU B: Chemical and Biological Sciences 57 (3 (262): 214-21. https://doi.org/10.46991/PYSU:B/2023.57.3.214.

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