ANTIOXIDANT PROPERTIES AND BINDING INTERACTIONS OF 1-MORPHOLIN-4-YL-3-{[2-(3-NITROPHENYL)-2-OXOETHYL]THIO}-5,6,7,8-TETRAHYDROISOQUINOLINE-4-CARBONITRILE WITH BOVINE SERUM ALBUMIN

Authors

  • Karine R. Grigoryan Laboratory of Physical Chemistry, Chemistry Research Center, YSU, Armenia https://orcid.org/0000-0001-6201-6540
  • Ani V. Igityan Laboratory of Physical Chemistry, Chemistry Research Center, YSU, Armenia
  • Heghine H. Vardanyan Scientific Technological Center of Organic and Pharmaceutical Chemistry of NAS of the RA, Institute of Fine Organic Chemistry of A.L. Mnjoyan, Armenia
  • Zhenya G. Khachatryan A. B. Nalbandyan Institute of Chemical Physics, NAS of the RA, Armenia https://orcid.org/0009-0006-3340-8901
  • Arpine S. Hatutyunyan Scientific Technological Center of Organic and Pharmaceutical Chemistry of NAS of the RA, Institute of Fine Organic Chemistry of A.L. Mnjoyan, Armenia https://orcid.org/0000-0002-6089-8727
  • Ashkhen L. Zatikyan Laboratory of Physical Chemistry, Chemistry Research Center, YSU, Armenia https://orcid.org/0000-0003-4765-5234
  • Hasmik A. Shilajyan Laboratory of Physical Chemistry, Chemistry Research Center, YSU, Armenia https://orcid.org/0000-0003-2329-2977

DOI:

https://doi.org/10.46991/PYSUB.2025.59.3.057

Keywords:

tetrahydroisoquinoline-4-carbonitrile, antioxidant, DPPH radical, bovine serum albumin, UV/Vis, FTIR

Abstract

The binding interactions of newly designed/synthesized 1-morpholin-4-yl-3- {[2-(3-nitrophenyl)-2-oxoethyl]thio}-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (C1) with transporting protein bovine serum albumin (BSA) were studied using UV/Vis and FTIR spectroscopy methods. The antioxidant properties of C1 were evaluated using DPPH˙  assay. FTIR spectra deconvolution of BSA amide I band (1600–1700 cm−1) was used to analyze changes in the secondary structure of BSA upon binding with C1. It was shown that C1 binds to BSA reversibly with KB = (3.52 ± 0.01)‧105 M–1 and the binding induces pronounced changes in the secondary structure of BSA. The obtained results demonstrate that C1 can be characterized as a compound with high radical (DPPH˙ ) scavenging capacity.

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Published

2025-12-01

Issue

Vol. 59 No. 3 (267) (2025)

Section

Chemistry

License

Copyright (c) 2025 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

“ANTIOXIDANT PROPERTIES AND BINDING INTERACTIONS OF 1-MORPHOLIN-4-YL-3-{[2-(3-NITROPHENYL)-2-OXOETHYL]THIO}-5,6,7,8-TETRAHYDROISOQUINOLINE-4-CARBONITRILE WITH BOVINE SERUM ALBUMIN”. 2025. Proceedings of the YSU B: Chemical and Biological Sciences 59 (3 (267): 57-66. https://doi.org/10.46991/PYSUB.2025.59.3.057.