BINDING MECHANISMS OF METHYLENE BLUE AND HOECHST 33258 WITH DNA

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2015.49.3.029

Keywords:

DNA ligand, Hoechst 33258, methylene blue, intercalation, semi-intercalation

Abstract

In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It was shown that Hoechst 33258 was bound to DNA by two types – “strong” and “weak”. It was also shown that the “strong” type of Hoechst 33258 binding depends on solution ionic strength.

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Published

2015-11-20

How to Cite

Antonyan, A.P. 2015. “BINDING MECHANISMS OF METHYLENE BLUE AND HOECHST 33258 WITH DNA”. Proceedings of the YSU B: Chemical and Biological Sciences 49 (3 (238):29-34. https://doi.org/10.46991/PYSU:B/2015.49.3.029.

Issue

Vol. 49 No. 3 (238) (2015)

Section

Biology

License

Copyright (c) 2015 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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