STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.A. Parsadanyan Chair of Biophysics, YSU, Armenia
  • V.G. Sahakyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSUB.2017.51.2.100

Keywords:

DNA, ethidium bromide, methylene blue, acridine orange, differential spectra, isosbestic point, pseudoisosbestic point

Abstract

Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and AO by intercalation and electrostatic modes. It was shown that the simultaneous performance of intercalation and semiintercalation modes condition the formation of a real isosbestic point (IP) in the spectra of EtBr–DNA complexes. In the absence of intercalation at MB interaction with DNA a pseudo-IP in differential absorption spectra of those complexes is formed. In the spectra of AO–DNA complexes both IP and pseudo-IP are not formed, despite the fact that this ligand binds to DNA by intercalation mode.

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Published

2017-06-13

Issue

Section

Biology

How to Cite

“STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA”. 2017. Proceedings of the YSU B: Chemical and Biological Sciences 51 (2 (243): 100-105. https://doi.org/10.46991/PYSUB.2017.51.2.100.

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