STUDY OF PECULIARITIES OF DIFFERENTIAL ABSORPTION SPECTRA OF COMPLEXES OF ACRIDINE ORANGE, ETHIDIUM BROMIDE AND METHYLENE BLUE WITH DNA
DOI:
https://doi.org/10.46991/PYSU:B/2017.51.2.100Keywords:
DNA, ethidium bromide, methylene blue, acridine orange, differential spectra, isosbestic point, pseudoisosbestic pointAbstract
Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and AO by intercalation and electrostatic modes. It was shown that the simultaneous performance of intercalation and semiintercalation modes condition the formation of a real isosbestic point (IP) in the spectra of EtBr–DNA complexes. In the absence of intercalation at MB interaction with DNA a pseudo-IP in differential absorption spectra of those complexes is formed. In the spectra of AO–DNA complexes both IP and pseudo-IP are not formed, despite the fact that this ligand binds to DNA by intercalation mode.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Proceedings of the YSU
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.